[ace ultracore superc18色谱柱]人参皂甙分析conditions
column: ace ultracore 2.5 superc18
dimensions: 100 x 3.0 mm; 2 x 100 x 3.0 mm (coupled); 3 x 100 x 3.0 mm (coupled)
part number: core-25a-1003u
流动相：
a: 0.1% formic acid in h2o
b: 0.1% formic acid in mecn
gradient:
time (mins)
100x3.0mm
200x3.0mm
300x3.0mm
%b
-
0.00
0.00
5
0.00
0.36
0.71
5
10.00
20.36
30.71
70
11.00
22.36
33.71
95
13.00
26.36
39.71
95
14.00
28.36
42.71
5
22.00
44.36
66.71
5
flow rate: 0.8 ml/min
injection: 2 μl (100 x 3.0 mm); 4 μl (200 x 3.0 mm); 6 μl (300 x 3.0 mm)
temperature: 80 °c
detection: uv, 203 nm
sample: 5 x 75 mg tablets ground to fine powder and extracted with 10.0 ml mecn/h2o (1:1 v/v) for 15 minutes with ultrasonication. 100 μl supernatant diluted with 300 μl water and filtered using a whatman mini-uniprep syringeless filter
system: chromaster ultra rs