nmt作为生命科学底层核心技术，是建立活体创新科研平台的*技术。2005年~2020年，nmt已扎根中国15年。2020年，中国nmt销往瑞士苏黎世大学，正式打开欧洲市场。
研究使用平台：nmt林木耐旱创新科研平台
期刊：new phytologist
主题：转录因子pdnf-yb21调控杨树根系生长并影响抗旱性
标题：the root-specific nf-y family transcription factor, pdnf-yb21, positively regulates root growth and drought resistance by aba-mediated iaa transport in populus
影响因子：7.299
检测指标：h+
检测样品：杨树根
h+流实验处理方法：
杨树茎段在hoagland营养液/hoagland营养液+0.1μm aba/hoagland营养液+10μm flu培养30天后检测根。
h+流实验测试液成份：未写明
作者：北京林业大学夏新莉、周扬颜
中文摘要（谷歌机翻）
根系生长控制在植物对干旱胁迫的适应中起着重要作用，但是这种控制的潜在分子机制仍然很难捉摸。
在此，从胡杨中分离出根特异性核因子y（nf-y）转录因子pdnf-yb21。pdnf-yb21的功能机制通过各种形态，生理，分子，生化和光谱学技术进行了表征。
杨树中pdnf-yb21的过表达促进木质部血管高度木质化和扩大的根系生长，从而增加了抗旱性。相反，crispr / cas9介导的杨树突变体nf-yb21表现出降低的根系生长和抗旱性。pdnf-yb21与b3域转录因子pdfusca3（pdfus3）相互作用。pdfus3直接激活aba合成关键基因pdnced3的启动子，导致水分缺乏的杨树根aba含量显着增加。杨nf-yb21和fus3的共表达显着增强了pdnced3的表达。
此外，aba促进了iaa在根尖的转运，终增加了根的生长和抗旱性。
英文摘要
root growth control plays an important role in plant adaptation to drought stress, but the underlying molecular mechanisms of this control remain largely elusive.
here, a root-specific nuclear factor y (nf-y) transcription factor pdnf-yb21 was isolated from populus. the functional mechanism of pdnf-yb21 was characterized by various morphological, physiological, molecular, biochemical, and spectroscopy techniques.
overexpression of pdnf-yb21 in poplar promoted root growth with highly lignified and enlarged xylem vessels, resulting in increased drought resistance. in contrast, crispr/cas9-mediated poplar mutant nf-yb21 exhibited reduced root growth and drought resistance. pdnf-yb21 interacted with pdfusca3 (pdfus3), a b3 domain transcription factor. pdfus3 directly activated the promoter of the aba synthesis key gene pdnced3, resulting in a significant increase in root aba content in poplars subjected to water deficit. coexpression of poplar nf-yb21 and fus3 significantly enhanced the expression of pdnced3.
furthermore, aba promoted iaa transport in root tips, which ultimately increased root growth and drought resistance.