果汁中维生素的快速液相色谱-质谱分析vitamins in fruit juice by fast lc-ms
conditions
column: ace excel 3 c18-pfp
dimensions: 100 x 2.1 mm
part number: exl -1110-1002u
mobile phase: a: 15 mm formic acid, adjusted to ph 3.8 with ammonia solution
b: meoh
gradient:
time(mins)
%b
0.00
1
1.00
1
3.00
8
3.10
25
6.00
50
6.50
50
6.51
1
9.00
1
flow rate: 0.4 ml/min
temperature: 30 °c
detection: lcms-8040 triple quad ms esi positive mode (esi negative for ascorbic and citric acid)
dl temperature: 250 °c
heat block temperature: 400 °c
analytes
1. thiamine (vitamin b1)(m/z 266.10 122.15)
2. pyridoxine (vitamin b6)(m/z 170.20 152.15)
3. nicotinic acid (vitamin b3)(m/z 124.00 78.00)
4. pantothenic acid (vitamin b5)(m/z 220.30 90.05)
5. cyanocobalamin (vitamin b12)(m/z 678.50 147.05)
6. riboflavin (vitamin b2)(m/z 377.20 243.10)
7. biotin (vitamin b7)(m/z 245.10 227.05)
8. nicotinamide (vitamin b3)(m/z 123.20 80.05)
9. ascorbic acid (vitamin c)(m/z 175.10 114.80)
10. citric acid(m/z 191.10 87.15)