圣约翰麦汁色谱分析st john’s wort
conditions
column: ace excel 1.7 superc18
dimensions: 100 x 3.0 mm; 2 x 100 x 3.0 mm (coupled); 3 x 100 x 3.0 mm (coupled)
part number: exl -1711-1003u
mobile phase:
a: 0.1% formic acid in h2o
b: 0.1% formic acid in mecn
gradient:
time (mins)
100x3.0mm
200x3.0mm
300x3.0mm
%b
-
0.00
0.00
5
0.00
0.35
0.71
5
15.00
30.35
45.71
30
17.00
34.35
51.71
100
19.00
38.35
57.71
100
20.00
39.35
58.71
5
27.00
53.35
79.71
5
flow rate: 0.8 ml/min
injection: 2 μl (100 x 3.0 mm); 4 μl (200 x 3.0 mm); 6 μl (300 x 3.0 mm)
temperature: 80 °c
detection: uv, 280 nm
sample: tablet ground to fine powder and extracted with mecn/h2o (1:1 v/v) with ultrasonication. supernatant diluted with h2o and filtered using whatman mini-uniprep syringeless filter
system: chromaster ultra rs