咖啡茵和代谢物分析
caffeine and metabolites
conditions
column: ace 5 superc18
dimensions: 150 x 4.6 mm
part number: exl-1211-1546u
mobile phase:
a: 20 mm ammonium acetate ph 7.0 in h2o
b: 20 mm ammonium acetate ph 7.0 in mecn/h2o (90:10 v/v)
time(mins)
%b
0
2
45
15
48
15
49
2
post time 10 minutes
flow rate: 1 ml/min
injection: 1 μl
temperature: 60 °c
detection: uv, 273 nm