epicypher新品 | 核酸提取缓冲液
cutana™ 细胞核提取缓冲液是cut&run和cut&tag实验中，用于从培养的细胞和组织中提取细胞核的必需试剂。这种缓冲液可与多种真核细胞和组织兼容。以下是使用该缓冲液成功进行细胞核分离的细胞和组织类型：
mouse nih3t3 fibroblast cells
human a549 non-small cell lung cancer (nsclc) cells
human k562 leukemia cells
human nci-h1299 non-small cell lung cancer (nscls cells)
human bone marrow derived macrophages
human tig-1 fetal lung cells
human monocyte derived macrophages
human lovo colorectal cancer cells
human mv-4-11 macrophage cells
human lncap prostate carcinoma cells
human sum149 triple negative breast cancer (tnbc) cells
human renal primary cells
human gm24385 b-lymphocyte (aka hg002) cells
human peripheral blood mononuclear cells (pbmcs)
human mcf7 breast cancer cells
human intestinal tissue
human mda-mb-231 breast cancer cells
human hepm embryonic cells
human sk-mel-2 melanoma cells
只需将试剂盒中的pre-nuclei extraction buffer和亚精胺与蛋白酶抑制剂混合即可完成该试剂的制备。cutana™ 细胞核提取缓冲液针对cut&run和cut&tag实验流程进行了优化，从而获得高质量的细胞核，助力您的基因组图谱分析。
试剂盒组成
组分
货号
cutana™pre-nuclei extraction buffer
21-1026a
1 m spermidine
21-1026b
保存条件
将pre-nuclei extraction buffer 4ºc储存, 1 m亚精胺-20ºc储存。
自收到之日起可稳定储存6个月。
验证数据
figure 1: nuclei extraction
nuclei were extracted from 3 different cell types: k562, nih3t3, and lncap using the cutana™ nuclei extraction protocol for cut&run and cut&tag. top 3 panels show cells before extraction. starting cells are viable (bright white and round). bottom 3 panels show nuclei extracted using the cutana™ nuclei extraction buffer as indicated by positive trypan blue staining.
订购详情
货号
产品名称
规格
21-1026
cutana™ nuclei extraction buffer
100 reactions
如需了解更多详细信息或相关产品，请联系epicypher中国授权代理商-欣博盛生物