艾美捷polymag crispr试剂盒#pnc40200高转染效率无毒性，灵敏性高、特异性强，用途范围广，具体特色如下：
• 即使在原代细胞和难以转染的细胞上也能高效递送 crispr/cas9 系统• 非常适合共转染 pdna/pdna、pdna/grna、pdna/mrna
• 针对含有 cas9 的大质粒 dna 进行了优化，并引导 rna
• 低细胞毒性 - 能够以更少的细胞开始实验
polymag crispr kit is the only magnet assisted transfection reagent optimized to deliver high level of plasmid dna and/or mrna expressing cas9 and guide rna (grna). this reagent is based on our proprietary magnetofection™ technology. the nanoparticles are composed of iron oxide magnetic core coated with cationic molecules. for your gene editing experiments, this reagent provides high transfection efficiency without toxicity.
benefits:
• high efficiency delivery of crispr/cas9 system even on primary and hard to transfect cells
• ideal for co-transfection of pdna/pdna, pdna/grna, pdna/mrna
• optimized for large plasmid dna containing cas9, and guide rna
• low cell toxicity - enable to start your experiment with less cell
用于crispr cas9实验的其他产品：
viromag crispr提高crispr/cas9病毒的转导效率
rmesfect crispr 用于 mrna 转染
prodeliverin crispr用于 cas9 蛋白递送
储存：+4°c
运输条件：室温
figure1 : polymag crispr co-transfection efficiency for crispr-cas9 experiments. nih-3t3, hela and mdck cells were plated in 24-well plates in complete medium at the optimum density of 50-70% confluence at the time of transfection. cells were transfected with a gfp-cas9 plasmid and a rfp pdna and then incubated overnight at 37oc. gfp and rfp expression was monitored 24h after transfection by fluorescence microscopy.