文献名：metaloxidenanoparticlesinduceuniqueinflammatoryfootprintsinthelung:importantimplicationsfornanoparticletesting
作者：wan-seobcho1,rodgerduffin1,craiga.poland1,sarahe.m.howie2,williammacnee1,markbradley3,ianl.megson4,andkendonaldson1
1elegi(theedinburghlungandtheenvironmentgroupinitiative),centreforinflammationresearch,universityofedinburgh,edinburgh,unitedkingdom;
2immunologygroup,centreforinflammationresearchand
3schoolofchemistry,universityofedinburgh,edinburgh,unitedkingdom;
4freeradicalresearchfacility,departmentofdiabetesandcardiovascularscience,centreforhealthscience,universityofthehighlandsandislands,inverness,unitedkingdom
摘要：
背景：metaloxidenanoparticles(nps)havebeenwidelyusedinindustry,cosmetics,andbiomedicine.
目的：weexaminedhazardsofseveralwell-characterizedhighproductionvolumenpsbecauseofincreasingconcernaboutoccupationalexposureviainhalation.
方法：apanelofwell-characterizednps[ceriumoxide(ceo2np),titaniumdioxide(tio2np),carbonblack(cbnp),silicondioxide(sio2np),nickeloxide(nionp),zincoxide(znonp),copperoxide(cuonp),andamine-modifiedpolystyrenebeads]wasinstilledintolungsofrats.weevaluatedtheinflammationpotenciesofthesenps24hrand4weekspostinstillation.fornpsthatcausedsignificantinflammationat24hr,wetheninvestigatedthecharacteristicsoftheinflammation.allexposureswerecarriedoutatequal-surface-areadoses.
结果：onlyceo2np,nionp,znonp,andcuonpwereinflammogenictothelungsofratsatthehighdosesused.strikingly,eachoftheseinducedauniqueinflammatoryfootprintbothacuy(24hr)andchronically(4weeks).acuy,patternsofneutrophilandeosinophilinfiltratesdifferedafterceo2np,nionp,znonp,andcuonptreatment.chronicinflammatoryresponsesalsodifferedafter4weeks,withneutrophilic,neutrophilic/lymphocytic,eosinophilic/fibrotic/granulomatous,andfibrotic/granulomatousinflammationbeingcausedrespectivelybyceo2np,nionp,znonp,andcuonp.
结论：differenttypesofinflammationimplydifferenthazardsintermsofpathology,risks,andriskseverity.invitrotestingcouldnothavedifferentiatedthesecomplexhazardoutcomes,andthishasimportantimplicationsfortheglobalstrategyfornphazardassessment.ourresultsdemonstratethatnpscannotbeviewedasasinglehazardentityandthatriskassessmentshouldbeperformedseparayandwithcautionfordifferentnps.
关键词：eosinophilicinflammation,intratrachealinstillation,invitroassay,invivoassay,lymphocyticinflammation,metaloxidenanoparticles,neutrophilicinflammation,riskassessment,surfaceareadose,wistarrat