lc-ms/ms法测定全氟酸perfluoro acids by lc-ms/ms
ace excel 2 c18 2μm, 50 x 2.1mm
gradient analysis
a = 2mm nh4oac, 0.1% acetic acid/ch3cn (95:5)
b = 2mm nh4oac, 0.1% acetic acid/ch3cn (5:95)
t (mins) % b t (mins) %b
0 25 7.5 95
0.5 25 8.0 25
5.5 95 10.0 25
flow rate: 0.5ml/min
column temperature: 40°c
injection volume: 20μl
ab sciex triple quad 5500
negative esi mrm
source temperature: 450°c
ionspray voltage: -2400v
peak id analyte
1 heptafluorobutyric acid
2 perfluorohexanoic acid
3 perfluorobutylsulphonic acid
4 perfluoroheptanoic acid
5 perfluorooctanoic acid
6 perfluorohexylsulphonic acid
7 perfluorononanoic acid
8 perfluorodecanoic acid
9 perfluorooctanesulphonic acid