饮用水中蓝藻/绿藻微囊藻毒素色谱分析microcystins from blue/green algae in drinking water
bruker advance uhplc system
ace excel 2μm c18, 100 x 2.1mm
gradient elution
a = 0.1% formic acid in water
b = acetonitrile
t (mins) %b t (mins) %b
0 30 7.1 30
1 30 10 30
7 95
flow rate: 0.4ml/min
column temperature: 40°c
injection volume: 50μl
concentration each microcystin: 0.05ppb
bruker evoq elite triple quad ms
vip heated-esi temperature: 350°c
cone gas temperature: 200°c
spray voltage: 4500v (+)
collision gas: argon 1.5mtorr