香兰素色谱分析vanillins
conditions
column: ace excel 3 c18-amide
dimensions: 150 x 4.6 mm
part number: exl -1112-1546u
mobile phase: a: 0.1% formic acid in h2o
b: 0.1% formic acid in mecn
gradient:
time(mins)
%b
0.0
30
10.0
55
10.5
55
15.0
30
20.0
30
flow rate: 1 ml/min
injection: 5 μl
temperature: 40 °c
detection: uv, 260 nm
analytes
1. vanillic acid
2. 4-hydroxybenzoic acid
3. vanillin
4. 4-hydroxybenzaldehyde
5. guaiacol
6. ethyl vanillin
7. eugenol