维生素-水溶性（梯度i）vitamins – water soluble (gradient i)
conditions
column: ace 5 c8
dimensions: 250 x 4.6 mm
part number: ace-122-2546
mobile phase: a: 50 mm kh2po4 ph
2.5 in h2o
b: meoh
gradient:
time(mins)
%b
0.0
0
3.0
0
16.5
45
19.5
80
flow rate: 1 ml/min
temperature: ambient
detection: uv, 205 nm
analytes
1. pyridoxamine
2. thiamine (vitamin b1)
3. l -ascorbic acid (vitamin c)
4. niacinamide (vitamin b3)
5. nicotinic acid
6. pyridoxal
7. pyridoxine
8. p-aminobenzoic acid
9. pantothenic acid (vitamin b5)
10. folic acid (vitamin b9)
11. cyanocobalamin (vitamin b12)
12. riboflavin (vitamin b2)
13. d-biotin (vitamin b7)
14. thioctic acid