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中英文说明丨艾美捷GMP IL-1β#C01002

interleukin-1 β (il-1 β) also known as endogenous mediators of white blood cells, monocytes, lymphocyte activating factors, etc., it is a cytokine protein encoded by the il1b gene in humans. interleukin-1 (il-1) has two genes: il-1 α and il-1 β (this gene). il-1 β precursor cytoplasmic caspase 1 (interleukin-1 β transformase cleavage to form mature il-1 β。
croyez aimejie gmp il-1 β# c01002 details:
source labeled with polyhistidine
escherichia coli
no animal reagents and laboratories
according to gmp guidelines
manufacturing and testing of endotoxin levels
every 0 through the lal method μ g protein<1.1 eu.
activity
measured by its ability to induce il-29 secretion in ht8 cells. the effect of education department 50 is<2.0 nanograms per milliliter.
recombinant il-1 β the specific activity is about>3 × 107iu/mg.
purity
>98% was determined by sds-page. purified by ni nta chromatography.
formula
freeze dry the protein from a solution containing 1x pbs and ph 8.0.
re dissolution is recommended in sterile h
freeze dried protein 2o to a concentration not less than 100 μ g/ml and incubate the stock solution for at least 20 minutes to ensure sufficient redissolution.
freeze dried protein should be stored
at -20 ° c. this product can be stably stored for one year upon receipt, and processed and stored according to the instructions. after re dissolution, the protein sample should be stored at -20 ° c or -80 ° c. avoid repeated freeze-thaw cycles.
be careful
please use it within one month after protein re dissolution.
croyez艾美捷gmp il-1β#c01002详细信息:
活性
通过其在ht8细胞中诱导il-29分泌的能力来衡量。教育署50这种效果是<2.0纳克/毫升。
重组人il-1β的比活性约为>3×107iu/mg.
纯度
>98% 由 sds-page 测定。通过ni-nta色谱法纯化。
配方
将蛋白质从含有1x pbs,ph 8.0的溶液冻干。
复溶建议在无菌h中复溶
冻干蛋白2o至浓度不低于100μg/ ml,并将储备溶液孵育至少20分钟以确保充分重新溶解。
储存冻干蛋白应储存
在-20°c。 本产品在收到后可稳定保存一年,并按照说明进行处理和储存。复溶后,蛋白质等分试样应储存在-20°c或-80°c。 避免反复冻融循环。
注意
请在蛋白质复溶后一个月内使用完毕。
gmp il-1β相关研究:
→ il-1β(白细胞介素-1β),人【c01002】
→ croyez gmp il-4(白细胞介素-4),人【c01006-gmp】
→ il-4(白细胞介素-4),人【c01006】
→ croyez gmp tgfβ1(转化生长因子β1),人【c01088-gmp】
→ 转化生长因子β1,人类【c01088】
→ il-9(白细胞介素-9),人【c01011】
→ croyez gmp il-21(白细胞介素-21),人【c01026-gmp】
→ il-21(白细胞介素-21),人【c01026】
→ croyez gmp干扰素-γ(干扰素-γ),人【c01080-gmp】
→ 干扰素-γ(干扰素γ),人类【c01080】
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