online coupling of microchip electrophoresis with ion mobility spectrometry for direct analysis of complex liquid samples
芯片电泳与离子迁移谱在线耦合，可直接分析复杂的液体样品
marián masára,? , jasna hradskia , michaela novákováa , roman szucsa , martin sabob , štefan matej?íkb
a department of analytical chemistry, faculty of natural sciences, comenius university in bratislava, mlynská dolina ch2, ilkovi?ova 6, 842 15, bratislava, slovakia
b department of experimental physics, faculty of mathematics, physics and informatics, comenius university in bratislava, mlynská dolina f2, 842 48, bratislava, slovakia
abstract
this is the first report of coupling of microchip electrophoresis (mce) with ion mobility spectrometry (ims) for the analysis of liquid samples. zone electrophoresis, employed on a microchip as a mce technique, is suitable for coupling with ims. sample components separated electrophoretically in liquid phase were transferred from the microchip using auxiliary liquid. direct liquid sampling interface was used for sample evaporation and introduction to ims. in the ims analyzer, the sample components were further separated in the gaseous phase. online mce-ims coupling enabled acquisition of characteristic ims response for the individual analytes. the proposed mce-ims technique was tested on a model mixture of a homologous series of carboxylic acids (formic acid, acetic acid, propionic acid, butyric acid, valeric acid and hexanoic acid) and subsequently applied to the analysis of wastewater sample obtained from a cattle farm. total analysis time did not exceed six minutes regardless of the sample. reproducibility of peak width in the mce ranged from 0.56 to 1.95% rsd, while reproducibility of time of ims response was in the range of 2.52–5.44% rsd for the studied carboxylic acids. rsd values of their reduced ion mobility were less than 0.56% for model and wastewater sample. limits of detection ranged from 0.07 to 2.61 mg l−1 . the results clearly show the great analytical potential of developed mce-ims coupling for the analysis of complex liquid samples.
这是微芯片电泳(mce)与离子迁移谱(ims)耦合用于液体样品分析的份报告。在微芯片上用作mce技术的区域电泳适用于与ims耦合。使用辅助液体，将通过电泳分离成液相的样品组分从微芯片中转移出来。直接液体采样接口用于样品蒸发和引入ims。在ims分析仪中，样品组分在气相中进一步分离。在线mce-ims耦合可获取单个分析物的特征ims响应。拟议的mce-ims技术在一系列羧酸的同源混合物(甲酸，乙酸，丙酸，丁酸，戊酸和己酸)，随后用于分析从养牛场获得的废水样品。无论样品如何，总分析时间均不超过六分钟。对于所研究的羧酸，mce中峰宽的重现性介于0.56至1.95%rsd之间，而ims响应时间的重现性介于2.52%至5.44%rsd之间。对于模型和废水样品，其降低的离子迁移率的rsd值小于0.56%。检测限范围为0.07至2.61 mg l 研究的羧酸的rsd为44%。对于模型和废水样品，其降低的离子迁移率的rsd值小于0.56%。检测限范围为0.07至2.61 mg l 研究的羧酸的rsd为44%。对于模型和废水样品，其降低的离子迁移率的rsd值小于0.56%。检测限范围为0.07至2.61 mg l-1。结果清楚地表明，开发的mce-ims偶联剂对复杂液体样品的分析具有巨大的分析潜力。