lc-ms/ms检测酒精生物标志物alcohol biomarkers by lc-ms/ms
ace excel 1.7 c18 1.7μm, 100 x 2.1mm
gradient analysis
a = 1mm ammonium fluoride
b = acetonitrile
time (mins) %b time (mins) %b
0 0 2.0 100
0.5 20 4.0 100
1.5 20 4.5 0
flow rate: 0.4ml/min
column temperature: 40°c
injection volume: 1μl
ab sciex triple quad 5500
negative esi mrm
source temperature: 750°c
ionspray voltage: -4