文献名：surfactant-polymernanoparticlesenhancetheeffectivenessofanticancerphotodynamictherapy
作者：aymankhdair,brigittegerard,hiteshhanda,guangzhaomao,malathyp.v.shekhar,andjayanthpanyam
departmentofpharmaceuticalsciences,eugeneapplebaumcollegeofpharmacyandhealthsciences,waynestateuniversity,detroit,michigan48201,departmentofpharmaceutics,collegeofpharmacy,universityofminnesota,minneapolis,minnesota55455,breastcancerprogram,karmanoscancerinstitute,110eastwarrenavenue,detroit,michigan48201,departmentofchemicalengineeringandmaterialsscience,collegeofengineering,waynestateuniversity,detroit,michigan48202,anddepartmentofpathology,waynestateuniversityschoolofmedicine,detroit,michigan48201
摘要：photodynamictherapy(pdt)isapromisingtreatmentmodalityforcancer.pdtisbasedontheconceptthatphotosensitizers,whenexposedtolightofspecificwavelength,generatecytotoxicreactiveoxygenspecies(ros)capableofkillingtumorcells.theeffectivenessofpdthasbeenlimitedinpartbythelackofphotosensitizersthataccumulatesufficientlyintumorcellsandpooryieldofrosfromexistingphotosensitizers.inthisreport,weinvestigatedwhetheraerosolot-alginatenanoparticlescanbeusedasacarriertoenhancethetherapeuticefficacyofamodelphotosensitizer,methyleneblue.methyleneblueloadednanoparticleswereevaluatedforpdteffectivenessintwocancercelllines,mcf-7and4t1.encapsulationofmethyleneblueinnanoparticlessignificantlyenhancedintracellularrosproduction,andtheoverallcytotoxicityfollowingpdt.italsoresultedinhigherincidenceofnecrosis.greatereffectivenessofnanoparticlescouldbecorrelatedwithhigheryieldofroswithnanoparticle-encapsulatedmethyleneblue.further,treatmentoftumorcellswithnanoparticle-encapsulatedmethyleneblueresultedinsignificantnuclearlocalizationofmethylenebluewhilefreedrugtreatmentresultedinitsaccumulationmainlyintheendolysosomalvesicles.inconclusion,encapsulationofmethyleneblueinaerosolot-alginatenanoparticlesenhanceditsanticancerphotodynamicefficacyinvitro.increasedrosproductionandfavorablealterationinthesubcellulardistributioncontributetotheenhancedpdtefficacyofnanoparticle-encapsulatedphotosensitizer.
关键词：reactiveoxygenspecies;photosensitizer;cancertherapy;cellulardelivery;cytotoxicity