metabolomic and transcriptomic changes underlying cold and anaerobic stresses after storage of table grapes
鲜食葡萄采后低温和厌氧胁迫下代谢组学和转录组学变化
itay maoz1,2,3, mirko de rosso4, tatiana kaplunov1, antonio dalla vedova4, noa sela5,
riccardo flamini4, efraim lewinsohn2 & amnon lichter1
the currently accepted paradigm is that fruits and vegetables should be consumed fresh and that their quality deteriorates during storage; however, there are indications that some metabolic properties can, in fact, be improved. we examined the effects of low temperature and high-co2 conditions on table grapes, vitis vinifera l. cv. ‘superior seedless’. berries were sampled at harvest (t0) and after low-temperature storage for 6 weeks under either normal atmosphere conditions (tc) or under an o2 level of 5 kpa and elevated co2 levels of 5, 10 or 15 kpa (t5, t10, t15). accumulation of 10 stilbenes,including e-ε-viniferin, e-miyabenol c and piceatannol, significantly increased under tc treatment as compared to t0 or t15. sensory analysis demonstrated that elevated co2 elicited dose-dependent offflavor
accumulation. these changes were accompanied by an accumulation of 12 volatile metabolites, e.g., ethyl acetate and diacetyl, that imparted disagreeable flavors to fresh fruit. transcriptome analysis revealed enrichment of genes involved in pyruvate metabolism and the phenylpropanoid pathway. one of the transcription factors induced at low temperature but not under high co2 was vvmyb14, which regulates stilbene biosynthesis. our findings reveal the potential to alter the levels of targeted metabolites in stored produce through understanding the effects of postharvest treatments.
目前*的模式是，水果和蔬菜应新鲜食用，储存期间其质量会下降；然而，有迹象表明，某些代谢特性实际上可以得到改善。我们研究了低温和高二氧化碳条件对葡萄的影响。“超级无核”。在采摘时(t0)和在正常大气条件(tc)下或在氧气含量为5kpa和二氧化碳含量为5、10或15kpa(t5、t10、t15)的条件下低温贮藏6周后对浆果进行取样。与t0和t15相比，tc处理显著增加了10种二苯乙烯的积累，其中包括e-ε-乙烯苷、e-米亚贝诺尔c和皮eatannol。感官分析表明，二氧化碳浓度升高引起的异味呈剂量依赖性积累。这些变化伴随着12种挥发性代谢物的积累，如乙酸乙酯和双乙酰，它们给新鲜水果带来了不愉快的味道。转录组分析显示丙酮酸代谢和苯丙酸途径相关基因的丰富。vvmyb14是低温诱导而非高co2诱导的转录因子之一，它调节二苯乙烯的生物合成。我们的发现揭示了通过了解采后处理的效果来改变贮藏产品中目标代谢物水平的潜力。