高效液相色谱-紫外分光光度法测定内酯和羟基酸形式的他汀类药物statins in lactone and hydroxy acid forms by hplc-uv
conditions
column: ace excel 3 superc18
dimensions: 100 x 3.0 mm
part number: exl -1111-1003u
mobile phase: a: mecn
b: 5 mm ammonium acetate ph 4.5 in h2o a/b (i) 73:27 v/v (ii) 55:45 v/v
flow rate: (i) 0.4 ml/min (ii) 0.3 ml/min
injection: 20 μl
temperature: 40 °c
detection: uv, 238 nm
analytes
1. simvastatin hydroxy acid
2. 4,4-dichlorodiphenyl trichloroethane (i.s.)
3. simvastatin lactone
4. pravastatin hydroxy acid
5. griseofulvin (i.s.)
6. pravastatin lactone
simvastatin: r1, r2 = ch3
pravastatin: r1 = oh, r2 = h