anti-gpr30抗体使用说明书
form：abcam
catalog number : byk-1380r
quantity size : 0.1ml (1mg/ml,lyophilized or liquid.buffer=0.01m pbs,ph 7.4 with 10 mg/ml bsa and 0.1% sodium azide.reconstitute with 0.1ml sterile distilled water.)
background: g protein-coupled receptors (gprs, or gpcrs) contain 7 hydrophobic transmembrane domains embedded in hydrophilic intra- and extracellular loops and transduce a variety of hormone, endogenous peptide, and neurotransmitter signals into intracellular effects via g proteins. grp30 is a member of this family and is an orphan receptor. gpr30 expression has been reported in brain, breast carcinoma, blood, bone marrow, cns, heart, liver, lung, lymph node, placenta, and spleen. in brain, gpr30 is expressed as a 2.8 kb transcript in basal forebrain, frontal cortex, thalamus, hippocampus, caudate and putamen. however, unlike other known g protein-coupled receptors, gpr30 localizes to the endoplasmic reticulum, where it specifically binds estrogen and estrogen derivatives.
specificity :
anti-gpr30 is a rabbit polyclonal antibody unconjugated specific for gpr30 of human,mouse,rat,dog,horseuse for western blotting,elisa,immunoprecipitation and immunohistochemistryprotein g affinity chromatography purification, purity :>95%isotype: iggmol wt: 42kda
application :
western blotting=1:100-500 immunohistocry 1:100-500 elisa= 1:500-1000 ihc-p=1:100-500ihc-f=1:100-500optimal working dilutions must be determined by the end user.
storage: store at -20℃ for one year. avoid repeated freeze/thaw cycles. the lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. when reconstituted in sterile ph 7.4 0.01m pbs or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
important note: this product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.