bioxcell热销产品--recombimab anti-mouse pd-l1 (b7-h1) (d265a)
产品描述：
10f.9g2™-cp001单克隆抗体是原始10f.9g2™单克隆抗体的重组嵌合型抗体。可变结构域序列与原始10f.9g2™克隆号相同，但是恒定区序列已经从大鼠igg2b变为小鼠igg1。10f.9g2™-cp001单克隆抗体在fc片段中也含有d265a突变，使其无法与内源性fcγ受体结合。
10f.9g2™-cp001单克隆抗体与小鼠pd-l1（程序性死亡配体1，也称为b7-h1或cd274）反应。pd-l1是属于ig超家族的b7家族的i型跨膜蛋白，分子量为40kda。pd-l1在t淋巴细胞、b淋巴细胞、nk细胞、树突状细胞以及ifnγ刺激的单核细胞、上皮细胞和内皮细胞上表达。pd-l1与cd4和cd8胸腺细胞以及活化的t和b淋巴细胞和骨髓细胞上的受体pd-1结合。pd-l1与pd-1的结合导致抑制tcr介导的t细胞增殖和细胞因子产生。pd-l1被认为在肿瘤免疫逃逸中起着重要作用。诱导的pd-l1表达在许多肿瘤中很常见，并导致肿瘤细胞对cd8+t细胞介导的裂解的抗性增加。在黑色素瘤的小鼠模型中，可以通过用阻断pd-l1和pd-1之间相互作用的抗体进行治疗，暂时抑制肿瘤生长。10f.9g2™单克隆抗体已被证明可以阻断pd-l1和pd-1之间以及pd-l1和b7-1之间的相互作用（cd80）。
产品详情：
产品名称
recombimab anti-mouse pd-l1 (b7-h1) (d265a) /欣博盛生物
产品货号
cp001
产品规格
1mg
反应种属
mouse
克隆号
10f.9g2™-cp001
同种型
mouse igg1(switched from rat igg2b)
免疫原
mouse cd274
实验应用
in vivo pd-l1 blockade*
immunofluorescence*
immunohistochemistry (frozen)*
flow cytometry*
western blot*
*reported for the original rat igg2b 10f.9g2 antibody
产品形式
pbs, ph 7.0,contains no stabilizers or preservatives
纯度
>95%, determined by sds-page
聚合
<5%, determined by sec
无菌处理
0.2 µm filtration
纯化方式
protein a
分子量
150 kda
小鼠病原检测
ectromelia/mousepox virus: negative
hantavirus: negative
k virus: negative
lactate dehydrogenase-elevating virus: negative
lymphocytic choriomeningitis virus: negative
mouse adenovirus: negative
mouse cytomegalovirus: negative
mouse hepatitis virus: negative
mouse minute virus: negative
mouse norovirus: negative
mouse parvovirus: negative
mouse rotavirus: negative
mycoplasma pulmonis: negative
pneumonia virus of mice: negative
polyoma virus: negative
reovirus screen: negative
sendai virus: negative
theiler’s murine encephalomyelitis: negative
保存条件
抗体原液保存在4°c，不能冷冻保存。
推荐抗体稀释液
invivopure ph 7.0 dilution buffer（货号ip0070）
该产品自上市已被多篇sci文献引用，品质有保证，以下是部分已发表的文献引用：
应用
文章
体内pd-l1阻断
(in vivo pd-l1 blockade)
1.grasselly, c., et al. (2018). 'the antitumor activity of combinations of cytotoxic
chemotherapy and immune checkpoint inhibitors is model-dependent' front
immunol 9: 2100.
2.stathopoulou, c., et al. (2018). 'pd-1 inhibitory receptor downregulates asparaginyl
endopeptidase and maintains foxp3 transcription factor stability in induced regulatory
t cells' immunity 49(2): 247-263 e247.
3.jaworska, k., et al. (2015). 'both pd-1 ligands protect the kidney from ischemia
reperfusion injury' j immunol 194(1): 325-333.
4.kim, j., et al. (2015). 'memory programming in cd8(+) t-cell differentiation is
intrinsic and is not determined by cd4 help' nat commun 6: 7994.
5.zander, r. a., et al. (2015). 'pd-1 co-inhibitory and ox40 co-stimulatory crosstalk
regulates helper t cell differentiation and anti-plasmodium humoral immunity' cell
host microbe 17(5): 628-641.
体内pd-l1阻断，流式细胞术
(in vivo pd-l1 blockade, flow
cytometry)
1.aloulou, m., et al. (2016). 'follicular regulatory t cells can be specific for the
immunizing antigen and derive from naive t cells' nat commun 7: 10579.
2.ngiow, s. f., et al. (2015). 'a threshold level of intratumor cd8+ t-cell pd1
expression dictates therapeutic response to anti-pd1' cancer res 75(18): 3800-3811.
3.rutigliano, j. a., et al. (2014). 'highly pathological influenza a virus infection is
associated with augmented expression of pd-1 by functionally compromised
virus-specific cd8+ t cells' j virol 88(3): 1636-1651.
体内pd-l1阻断，免疫荧光
(in vivo pd-l1 blockade,
immunofluorescence)
1.willimsky, g., et al. (2013). 'virus-induced hepatocellular carcinomas cause
antigen-specific local tolerance' j clin invest 123(3): 1032-1043.
免疫组织化学（冷冻），免疫荧光
(immunohistochemistry (frozen),
immunofluorescence)
1.riella, l. v., et al. (2011). 'essential role of pdl1 expression on nonhematopoietic
donor cells in acquired tolerance to vascularized cardiac allografts' am j transplant
11(4): 832-840.
更多产品详情请联系bioxcell中国授权代理——欣博盛生物