anti-eea1抗体价格
catalog number : byk-11250r
quantity size : 0.1ml (1mg/ml,lyophilized or liquid.buffer=0.01m pbs,ph 7.4 with 10 mg/ml bsa and 0.1% sodium azide.reconstitute with 0.1ml sterile distilled water.)
background: early endosomes are cytoplasmic compartments that function in receiving and sorting endocytosed proteins for vesicular transport. eea1 (early endosome antigen 1) is a peripheral membrane protein that co-localizes with the transferrin receptor and rab5 on early endosomes. eea1 contains a calmodulin-binding iq motif and cysteine rich finger motif necessary for its specific localization to the early endosomes. eea1 has sequence homology to several yeast proteins that have been implicated in membrane trafficking, including vps27, fab1 and vac1. evidence suggests a possible role for eea1 in mediating the regulatory effects of 3'-phosphoinositides on membrane trafficking.
specificity :
anti-eea1 is a rabbit polyclonal antibody unconjugated specific for eea1 of human,mouse,rat,dog,cow,hamsteruse for western blotting,elisa,immunoprecipitation and immunohistochemistryprotein g affinity chromatography purification, purity :>95%isotype: iggmol wt: 162kdaapplication :
western blotting=1:100-500 immunohistocry 1:100-500 elisa= 1:500-1000ihc-p=1:100-500ihc-f=1:100-500icc=1:100-500 if=1:100-500optimal working dilutions must be determined by the end user.
storage: store at -20℃ for one year. avoid repeated freeze/thaw cycles. the lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. when reconstituted in sterile ph 7.4 0.01m pbs or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
important note: this product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.