香兰素快速分析vanillins – fast analysis
conditions
column: ace excel 1.7 c18-amide
dimensions: 50 x 3.0 mm
part number: exl -1712-0503u
mobile phase: a: 0.1% formic acid in h2o
b: 0.1% formic acid in mecn
gradient:
time(mins)
%b
0.0
25
1.32
75
1.49
75
1.60
25
flow rate: 1.3 ml/min
injection: 1 μl
temperature: 45 °c
detection: uv, 260 nm
analytes
1. vanillic acid
2. 4-hydroxybenzoic acid
3. vanillin
4. 4-hydroxybenzaldehyde
5. guaiacol
6. o -vanillin
7. ethyl vanillin
8. eugenol