离子对lc-ms/ms法测定阿糖胞苷类似物分析cytarabine analogues by ion-pairing lc-ms/ms
ace 3 c18 3μm, 50 x 2.1 mm
gradient analysis
a = 0.1% perfluoropentanoicacid + 0.1% formic acid in water
b = 0.1% perfluoropentanoicacid + 0.1% formic acid in acetonitrile
t (mins) %b
0 0
0.5 0
3.0 13
4.0 90
5.0 0
flow rate: 0.7 ml/min
api 4000 ms
turboionspray, positive mode
source temperature: 550°c
cytarabineand cytidineare isobaric.
robust method with good separation achieved.
lloq = 1 ng/ml human plasma