anti-e2f3抗体热销
catalog number : byk-1722r
quantity size : 0.1ml (1mg/ml,lyophilized or liquid.buffer=0.01m pbs,ph 7.4 with 10 mg/ml bsa and 0.1% sodium azide.reconstitute with 0.1ml sterile distilled water.)
background: the protein encoded by this gene is a member of the e2f family of transcription factors. the e2f family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small dna tumor viruses. the e2f proteins contain several evolutionally conserved domains found in most members of the family. these domains include a dna binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (dp), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. this protein and another 2 members, e2f1 and e2f2, have an additional cyclin binding domain. this protein binds specifically to retinoblastoma protein prb in a cell-cycle dependent manner.
specificity :
anti- e2f3 is a rabbit polyclonal antibody unconjugated specific for e2f3 of human,mouse,rat,dog,pig,cow,horse,rabbit,use for western blotting,elisa,immunoprecipitation and immunohistochemistryprotein g affinity chromatography purification, purity :>95%isotype: iggmol wt: 49kda
application :
western blotting=1:100-500 immunohistocry 1:100-500 elisa= 1:500-1000if=1:100-500 ip=1:20-100ihc-p=1:100-500ihc-f=1:100-500optimal working dilutions must be determined by the end user.
storage: store at -20℃ for one year. avoid repeated freeze/thaw cycles. the lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. when reconstituted in sterile ph 7.4 0.01m pbs or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
important note: this product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.