lc-ms/ms法检测兽药类固醇veterinary steroids by lc-ms/ms
ace ultracore superc18, 2.5um, 100 x 2.1mm
waters acquity sds system
gradient analysis
a = 0.01mm amm. fluoride + 0.001% formic acid
b = acetonitrile
time (mins) %b time (mins) %b
0 25 7.5 35
0.5 25 10.5 60
7.0 35
flow rate: 0.5ml/min
column temperature: 45°c
positive or negative esi mrm data
also analysed in the same run (-esi):
taleranol and zeranol-d4
taleranol and zeranol
zearalenone
hexestrol
diethylstilbestrol
dienestrol
also analysed in the same run (+esi):
hydroxystanazolol
hydroxystanazolol-d3
methyltestosterone
methyltestosterone-d3
β-nortestosterone-d3
β-trenbolone
α-trenbolone