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期刊：food chemistry
主题：钙参与盐胁迫下作物酚类积累的gaba信号转导（钙信号）
标题：ca2+ involved in gaba signal transduction for phenolics accumulation in germinated hulless barley under nacl stress
影响因子：5.399
检测指标：ca2+流速
检测部位：根尖细胞
ca2+流实验处理方法：
三日龄的大麦幼苗，60mm nacl、60mm nacl + 0.5mm gaba、60mm nacl + 0.1mm 3-mp分别处理四天和六天
ca2+流实验测试液成份：
0.1mm kcl, 0.1mm cacl2, 0.1mm mgcl2, 0.5mm nacl, 0.3mm mes, 0.2mm na2so4，ph 6.0
通讯作者：南京农业大学杨润强、马燕
英文摘要
in this study, in order to investigate the role of ca2+ in gaba signal transduction involved in phenolics accumulation in barley seedlings under nacl stress, the seedlings were treated with exogenous gaba and its synthesis inhibitor, 3-mercaplopropionic acid (3-mp), as well as ca2+ channel blockers la3+, ca2+ chelator egta, and ca2+ release channel inhibitor 2-aminoethoxydiphenyl borate (2-apb).
the results showed that gaba significantly enhanced phenolics, calcium and calmodulin content. it also induced ca2+ influx in barley root tips cells, and altered the distribution of ca2+, making calcium precipitates more uniform and intensive. while, 3-mp treatment led to opposite changes, which suggested that gaba was essential for calcium content increase. in addition, accumulation of phenolics was inhibited by lacl3, egta and 2-apb treatments, and this inhibition could be alleviated partly by exogenous gaba.
taken together, ca2+ was involved in gaba signal transduction for phenolics accumulation in barley seedlings under nacl stress.
中文摘要（谷歌机翻）
在本研究中，为了研究ca2+在nacl胁迫下大麦幼苗中酚类物质积累的gaba信号转导中的作用，用外源gaba及其合成抑制剂3-巯基丙酸（3-mp）处理幼苗。以及ca2+通道阻滞剂la3+，ca2+螯合剂egta和ca2+释放通道抑制剂2-氨基乙氧基二苯基硼酸盐（2-apb）。
结果表明，gaba显着增强了酚类，钙和钙调蛋白的含量。它还诱导大麦根尖细胞内的ca2+流入，并改变ca2+的分布，使钙沉淀更均匀和密集。然而，3-mp处理导致相反的变化，这表明gaba对钙含量的增加至关重要。此外，lacl3，egta和2-apb处理抑制了酚类物质的积累，这种抑制作用可以通过外源性gaba部分缓解。
总之，ca2+参与了nacl胁迫下大麦幼苗中酚类物质积累的gaba信号转导。
结果表明，对照大麦幼苗根尖ca2+外排，与对照相比，nacl处理引起的ca2+由外排向吸收转变。gaba处理得到稳定的ca2+吸收，其中处理6天的吸收明显高于处理4天吸收。gad抑制剂（3-mp）在不同处理时期产生了不同的趋势，与单独使用nacl处理相比，第4天ca2+的外排速率显着增加，但第6天ca2+流入量较弱。
注：ck表示大麦用蒸馏水喷洒; n表示60mm nacl处理; ng表示60mm nacl + 0.5mm gaba处理; nm表示60mm nacl + 0.1mm 3-mp处理。